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Cv de ingenieur de recherche

Ingenieur de recherche/ chargé de projet scientifique/conseil genetique/eneignement

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CV Code: 5adf1d15dcf38882
Date of last connection: 2018-04-27

Mr. Zi... R...

....

paris 75019
France

Current situation:

Current industry: Recherche

Size of the company: 101-1000 employees

Current position: Post doctorant

Number of years spent at this position: 3-5 years

Number of persons you managed: 0

Annual salary: 26000.00 EUR

Total working experience: 3-5 years

Availability: Immediate availability

Job sought:

Positions: ingenieur de recherche, chargé de projet scientifique, conseil genetique

Industry: ile de france, ,

Desired type of contract: open-ended contract, fixed term contract, Fixed-term / casual work, Temporary work, Agent's contract

Desired working time: Full-time, Part-time, Study-work program, Seasonal work

Minimum annual salary desired: 30000.00 / 0.00 EUR

Education:

Highest educational level completed: number of years studied and completed with a diploma after highschool : +10

Last diploma : thése de coctorat en biologie moleculaire

Current educational level : +11

Other training courses :


Divers :

Geographical mobility: Country : France

Known Tools / Software/ Methods Genetic and molecular biology: DNA/RNA extraction, PCR, Electrophoresis, Automated sequencing, sequence analysis, Qpcr, Cloning, Transformation, Site directed mutagenesis, transfection with COS7 and 1 type, RT pcr, minigene assays to analyze the effect of atypical splice variants on splicing mechanism. Bioinformatics analysis: Analysis and comparison of sequences (NCBI, EBI, UCSC, Ensembl, Bioedit, DNASTAR, Sequencher, Alamut Fruitfly, EVS…), Analysis of NGS data (whole exome sequencing and targeted exome sequencing) (INTEGRAGEN). Biochemistry and microbiology: Cell culture, Protein Extraction, Protein Assay, Isolation of Bacteria from the soil, Optimisation of the growth factors for some microorganisms, modeling of the pigment production by the Streptomyces sp using SPSS software


HGV, LGV, special vehicles licences permis tunisien


Languages Arabic : Native
French : Fluent
English : Advanced


Resume:

Mr. Zi... R...

....

paris 75019
France

Ingénieur de recherche/ Chargé de
projet scientifique/ Attaché de recherche clinique/ Enseignement


EDUCATION

09/2011-09/2014 PhD in Human Genetics, Laboratory of
Biomedical Genomics and Oncogenetics, Pasteur Institute of Tunis, College of
Sciences, University of Tunis

09/2008- 12/2010 Master in Biochemistry and Biotechnology,
Laboratory of Biochemistry and Biotechnology

College of Sciences, University of Tunis, Tunisia

Tunisia 09/2004- 06/2008 Bachelor in Basic Sciences, College
of Sciences, University of Tunis, Tunisia

AWARDS & HONORS

2014/2013: International
Genomedika project scholarship

2014/2013 International Researcher’s scholarship of the Tunisian
Ministry of Higher Education and Scientific Research

2013 Mobidoc scholarship for graduate students of the National Agency of
Scientific Research financed by the European Union

2011/2012/2013: Young Researcher's Scholarship of the Tunisian Ministry
of Higher Education and Scientific Research

RESEARCH

02/2015-
until now: Post doc
researcher
in Institut de la Vision, INSERM UMRS 1120, Paris, France

Lab
director: Pr. Christine Petit-
Targeted Exome sequencing for molecular diagnosis of deafness and Usher genes
for maghrebian and European families.

11/2014
Institut de la Vision, INSERM UMRS 1120, Paris, France

Lab
director: Pr. Christine Petit-
Targeted Exome sequencing of deafness genes for Tunisian, Algerian, Moroccan
and Mauritanian families with Hearing Loss.

04/2014- 07/2014 Institut de la Vision, INSERM UMRS 1120, Paris, France

Lab
director: Pr. Christine Petit-
Targeted Exome Sequencing of deafness genes for 60 Tunisian families with
Hearing Loss.

10/2013- 12/2013 Unité de Génétique et Physiologie de l'Audition, Institut Pasteur
de Paris, UMRS 1120 and Institut de la Vision, INSERM UMRS 1120, Paris, France

Lab
director: Pr. Christine Petit -Whole
Exome Sequencing for families with Hereditary Hearing Loss and presbycusis and
investigation of the candidate variants

04/2013- 06/2013 Institut de la Vision, INSERM UMRS 1120, Paris, France

Lab
director: Pr. Christine Petit-
Molecular investigation of 51 Tunisian families with Hearing Loss

09/2011-09/2014: PhD researcher in Laboratory of
Biomedical Genomics and Oncogenetic. Pasteur Institute of Tunis, Tunisia

Lab
director: Pr. Sonia Abdelhak -
Determination of the molecular etiology of sensorineural hereditary hearing
loss in Tunisia: Implications for diagnosis, therapy and genetic counseling.

Member of
GENOMEDIKA team, an FP7 era-wide project entitled “Reinforcing IPT capacities
in Genomic Medicine, Non Communicable Diseases Investigation and International
Cooperation Activities” (FP7-INCO.2011-6.2, acronym GM_NCD_InCo
(genomedika.org) grant agreement N° 295097)

09/2008-
12/2010 Master
degree
in Laboratory of Biochemistry and Biotechnology, College of
Sciences, University of Tunis, Tunisia

Lab
director: Pr. Omrane Belhaj-
Bacterial Biochemistry: Modeling of the pigment production by strains of
Streptomyces.sp and optimization of its antimicrobial and antifungal activity.

SKILLS AND
COMPETENCES


Genetic and
molecular biology: DNA/RNA extraction, PCR,
Electrophoresis, Automated sequencing, sequence analysis, Qpcr, Cloning,
Transformation, Site directed mutagenesis, transfection with COS7 and 1 type,
RT pcr, minigene assays to analyze the effect of atypical splice variants on splicing
mechanism.

Bioinformatics
analysis: Analysis and comparison of sequences (NCBI, EBI,
UCSC, Ensembl, Bioedit, DNASTAR, Sequencher, Alamut Fruitfly, EVS…), Analysis
of NGS data (whole exome sequencing and targeted exome sequencing)
(INTEGRAGEN).

Biochemistry
and microbiology: Cell culture, Protein Extraction,
Protein Assay, Isolation of Bacteria from the soil, Optimisation of the growth
factors for some microorganisms, modeling of the pigment production by the
Streptomyces sp using SPSS software

PUBLICATIONS

1- Zied R, Hassen Hammami, Houyem
Ouragini, Habib Messai , Rim Zainine, Lilia Romdhane, Donia Essaid, Rym Kefi,
Mohsen Rhimi, Monia Bedoui, Delphine Feldmann, Laurence Jonard, Ghazi Besbes,
Sonia Abdelhak. Update of the spectrum of GJB2
gene mutations in Tunisian families with autosomal recessive nonsyndromic
hearing loss. Gene. 2013 Aug 1; 525(1):1-4.


2- Zied
R, Rim Zainine, Yosra Mellouli, Raja Hannachi, Yosra Bouyacoub, Nadia
Laroussi, Najeh Beltaief, Rym Kefi, Lilia Romdhane, Crystel Bonnet, Sonia
Abdelhak, Ghazi Besbes. Compound heterozygosity for dominant and recessive GJB2 mutations in a Tunisian family and
association with successful cochlear implant outcome. International journal of
pediatric otorhinolaryngology. 2013 Sep; 77(9):1481-4.

3- Zied R, Houda Chahed, Habib
Jaafoura, Rim zainine, Mohamed Naili, Hassan Hammami, Nadia Laroussi, Yosra
Bouyacoub, Rym Kefi, Crystel Bonnet, Ghazi Besbes, Sonia Abdelhak. A novel
frameshift mutation (c.405delC) in the GJB2
gene associated with autosomal recessive hearing loss in two Tunisian families.
International journal of pediatric otorhinolaryngology. 2013 Sep; 77(9):1485-8.


4- Zied R, Crystel Bonnet, Rim
Zainine, Malek Louha, Yosra Bouyacoub, Nadia Laroussi , Mariem Chargui , Rym
Kefi, Laurence Jonard, Imen Dorboz, Jean-Pierre Hardelin, Sihem Belhaj Salah ,
Jacqueline Levilliers, Dominique Weil, Kenneth McElreavey, Odile Tanguy
Boespflug, Ghazi Besbes, Sonia Abdelhak, Christine Petit. Whole exome
sequencing identifies new causative mutations in Tunisian families with
non-syndromic deafness. Plos One. 2014 Jun; 9(6): e99797.

5- Lilia
Romdhane, Nizar Ben Halim, Insaf Rejeb, Rym Kefi, Yosra Bouyacoub, Mariem Ben
Rekaya, Habib Messai, Olfa Messaoud, Zied
R, Crystel Bonnet, Faten Ben Rhouma, Majdi Nagara, Christine Petit, Ken
McElreavey , Giovanni Romeo, Sonia Abdelhak. Specific aspects of consanguinity:
some examples from the Tunisian population. Hum Hered. 2014 Jul;
77(1-4):167-74.

6-Nagara M,
Voskarides K, Elouej S, Zaravinos A, R ZIED, Papagregoriou G, Kefi R, Boussetta K, Deltas C, Abdelhak S, Tinsa F. A
novel splice-site mutation in ATP6V0A4
gene in two brothers with distal renal tubular acidosis from a consanguineous
Tunisian family. J Genet. 2014 Dec;93(3):859-63

7- Zied R , Bonnet C, Zainine R, Lahbib S,
Bouyacoub Y, Bechraoui R, Marrakchi J, Hardelin JP, Louha M, Largueche L, Ben
Yahia S, Kheirallah M, Elmatri L, Besbes G, Abdelhak S, Petit C. Whole exome
sequencing identifies mutations in Usher syndrome genes in profoundly deaf
Tunisian patients. PLoS One. 2015 Mar 23;10(3):e0120584. doi: 10.1371.

8-Delmaghani
S, Aghaie A, Bouyacoub Y, El Hachmi H, Bonnet C, Zied R , Chardenoux S, Perfettini I, Hardelin JP, Houmeida A,
Herbomel P, Petit C. Mutations in CDC14A,
Encoding a Protein Phosphatase Involved in Hair Cell Ciliogenesis, Cause
Autosomal-Recessive Severe to Profound Deafness. Am J Hum Genet. 2016 Jun
2;98(6)

9-Bonnet C, Zied R,
Chantot-Bastaraud S, Smagghe L, Letexier M, Marcaillou C, Lefèvre GM, Hardelin
JP, El-Amraoui A, Singh-Estivalet A, Mohand-Saïd S, Kohl S, Kurtenbach A,
Sliesoraityte I, Zobor D, Gherbi S, Testa F, Simonelli F, Banfi S, Fakin A,
Glavač D, Jarc-Vidmar M, Zupan A, Battelino S, Martorell Sampol L, Claveria MA,
Catala Mora J, Dad S, Møller LB, Rodriguez Jorge J, Hawlina M, Auricchio A,
Sahel JA, Marlin S, Zrenner E, Audo I, Petit C. An innovative strategy for

the molecular diagnosis of Usher syndrome identifies causal biallelic
mutations in 93% of European patients. Eur J Hum Genet. 2016 Jul 27.



10- Moctar
EC, Zied R , El Hachmi H, Veten F,
Meiloud G, Bonnet C, Abdelhak S, Errami M, Houmeida A. Etiology and associated GJB2 mutations in Mauritanian children
with non-syndromic hearing loss. Eur Arch Otorhinolaryngol. 2016 Apr 11.

11- Bousfiha
A, Bakhchane A, Charoute H, Zied R, Snoussi K, Rouba H, Bonnet C, Petit
C, Barakat A. A novel PEX1
mutation in a Moroccan family with Zellweger spectrum disorders. Hum Genome Var. 2017 Apr.

12- Zied R, Crystel
Bonnet, Yosra Bouyacoub, Aziz El Amraoui, Jean-Pierre Hardelin, Christine
Petit. Molecular diagnosis of hereditary hearing impairment
by high throughput sequencing of targeted exome in patients from North Africa
and Jordan. Eur J Hum Genet. Submitted.

13- Zied R., Saida Lahbib., Crystel
Bonnet., Melanie Letexier., Jihene Marrakchi., Rym Zainine., Ghazi Besbes., Sonia
Abdelhak., Christine Petit. Identification of two novel mutations in KARS gene associated with autosomal
recessive hearing loss in Tunisian families using targeted exome sequencing.
Gene. Submitted.

14- Marwa Sayeb, Zied R,
Nadia Laroussi, Crystel Bonnet, Lilia Romdhane, Rahma Mkaouar, Anissa Zaouak,
Jihene Marrakchi, Ghaith Abdessalem,
Haifa Jmel, Saida Lahbib, Olfa Messaoud, Mariem Chargui, Oussema Bouchniba,
Nacer Ghilane, Beatrice Regnault, Mourad Sahbatou, Thomas Bourgeron,
Anne-Louise Leutenegger, Mourad Mokni, Ghazi Besbes, Houda Yacoub, Christine Petit,
Sonia Abdelhak: Co-occurrence of ichthyosis and hearing loss: a phenocopy of
KID syndrome in a Tunisianconsanguineous family with a novel CYP4F22 mutation. Submitted.



PRESENTATIONS

• Molecular diagnosis of
hereditary prelingual severe to profound hearing impairment by high throughput
sequencing of targeted exome in patients from North Africa and Jordan. European
Society of Human Genetics. Copenhagen, Danemark. 27-31 May 2017- Poster
presentation.

• Improvement of molecular diagnosis
of hereditary hearing loss in families from North Africa and Middle East using
targeted exome sequencing (Hear panel). Scientific Symposium of the Institut
Pasteur International Network. Paris, November 29- December 2, 2016- Oral
Presentation.

• Usher
syndrome: an innovative strategy of molecular diagnosis identifies the causal
mutations in the vast majority of European patients. Scientific Day of vision
Institute of Paris September 11, 2015

• Etiology
of deafness in children candidates to cochlear implantation in Tunisia:
importance of molecular diagnosis. Scientific Symposium of the Institut Pasteur
International Network. Paris, September 10- 13, 2014- Poster Presentation.

• Whole
exome sequencing identifies new causative mutations in Tunisian families with
non-syndromic deafness. European Society of Human Genetics. Milan, Italy. May
31-June 3 2014- Poster presentation.

• Whole
exome sequencing identifies new causative mutations in Tunisian families with
non-syndromic deafness. 7th courses of Human and Medical Genetics, Bordeaux,
France - 29-31 January 2014- Poster presentation.

• Update of
the spectrum of GJB2 gene mutations in Tunisian families with autosomal
recessive nonsyndromic hearing loss (2013). European Society of Human Genetics.
Paris, France- Poster presentation.

• Modeling
and optimization of pigment production in strains of Streptomyces.sp (2010).The
20th meeting of the Tunisian Society of Natural Sciences. Tunisia - Oral
presentation.


Identification and characterization of the antimicrobial activity of
Streptomyces sp (2009).The 19thmeeting of the Tunisian Society of Natural
Sciences. Tunisia - Oral presentation.

COURSES

• International
Symposium on Inner Ear Therapies. Marrakech, Morroco, 2017.

• Symposium
on Consanguinity and hereditary rare diseases: challenges and perspectives in
post-genomics and Genomedika Final conference. Pasteur Institute of Tunis. 2014.



International Course on Functional Genomics- GENOMEDIKA. Pasteur Institute of
Tunis. 2014.


International genomic diversity and health of populations - GENOMEDIKA-1
course-Pasteur Institute of Tunis -2012.

• Molecular
and Statistical Genetics of Consanguinity. European Genetics Foundation.
Pasteur Institute of Tunis -2012.

• Illumina:
New technologies for sequencing. Applications of second-generation sequencers
and microarrays in the field of Research and Diagnostics - Pasteur Institute of
Tunis – 2012.

• Affymetrix
Technology: Systems and applications, Main Affymetrix Microarray applications.
Pasteur Institute of Tunis – 2012.

EXTRACURRICULAR
ACTIVITIES


• Football
player with Rades team (2010- 2013)


Physiotherapist for Ezzahra Handball team (2011-2012)

• Member of
the Red Crescent/Cross (2007- 2014)

HOBBIES
& INTERESTS


Football,
Handball, Music.

Cover letter

Mr. Zi... R...

....

paris 75019
France

Ingenieur de recherche/ chargé de projet scientifique/conseil genetique/eneignement

Dear Committee Focused upon entering into a lab research position, I’ve opted to forward my resume to you for perusal and action. I have the experience and educational background you are looking for an ideal candidate. I’m a postdoc researcher in Usher team headed by Pr Christine PETIT in Institut de la Vision of Paris. I’m working on the molecular diagnosis of isolated Hearing loss and Usher Syndrome using next generation sequencing coupled to targeted exome sequencing in Maghrebian and European populations population respectively. In addition im studying the effect of some atypical mutations using minigenes assay which is based on bacterial culture (cloning, transformation, site directed mutagenesis with E.Coli) Cell culture, transfection with COS7 and 1 type, RT pcr. My thesis work (2011-2014) focused on the determination of molecular etiology of pediatric non syndromic hearing loss in Tunisia and its implication in diagnostics, therapy and genetic counseling. In a first time, I have determined the the spectrum of GJB2 gene mutations in Tunisian families with autosomal recessive nonsyndromic hearing loss. After i was interested to the investigation of candidate variants using whole exome sequencing (Integragen). These studies were the subject of 13 publications in different scientific journals. In addition to my academic achievements and my research activities, i have developed strong leadership and communication skills: i supervised many students from collaborate countries on the field of Human Genetic during my thesis and my post doc studies; in addition i participate and present usually my work either in oral or poster presentation in several international congress. I was a Member of GENOMEDIKA team, an FP7 era-wide project entitled “Reinforcing IPT capacities in Genomic Medicine, Non Communicable Diseases Investigation and International Cooperation Activities. My curriculum and my professional experience in the field of genetics, rare disease, NGS and cell culture have given me the knowledge and the will to face the challenges, the questioning and the importance of the teamwork. I like to give myself deeply into everything I do and I know how to adapt to new and unexpected situations. In order to meet a new challenge and show you my motivation and enthusiasm I am sending you my application. I have attached a copy of my CV to this email for your review. I appreciate your consideration of this application and I would be happy to present a seminar on my work in any time. I look forward to hearing from you. Best regards,

Mr. Zi... R



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